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kam-880 microarray kit  (Kinex Pharmaceuticals)

 
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    Kinex Pharmaceuticals kam-880 microarray kit
    FOXO1 expression is reduced in HSC-5 NW cells. ( A ) Individual duplicate arrays of phospho-Ser319 FOXO1 for the HSC-5 CN (red) and HSC-5 NW (green) cell lysate protein microarrays; the complete Kinex <t>KAM-880</t> microarray is shown in . The average values of HSC-5 CN and HSC-5 NW cell phospho-Ser319 FOXO1 arrays are shown. ( B ) Representative Western blots of FOXO1 and GAPDH in HSC-5 CN and HSC-5 NW cells; n = 3. ( C ) Densitometric quantification of the FOXO1/GAPDH ratio in HSC-5 CN and HSC-5 NW cells normalized to HSC-5 CN cells. ( D ) Total RNA was extracted from HSC-5 CN and HSC-5 NW cells and reverse-transcribed to cDNA for quantifying FOXO1 expression relative to MRPL-27 using real-time PCR, normalized to HSC-5 CN cells. ( E ) Representative Western blots of FOXO1 and GAPDH in HSC-5 CN and HSC-5 NW cells treated with 10 µM MG132 or DMSO; n = 3. ( F ) Densitometric quantification of the FOXO1/GAPDH ratio in HSC-5 CN and HSC-5 NW cells treated as in ( E ) normalized to DMSO-treated HSC-5 CN cells. ( G ) Representative immunofluorescence images of HSC-5 CN and HSC-5 NW cells treated as in ( E ) stained for FOXO1 (green). Nuclei were counterstained with DAPI (blue). Scale bar represents 20 µm, n = 3. All values are the mean ± SD, n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001.
    Kam 880 Microarray Kit, supplied by Kinex Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kam-880+microarray+kit/pmc09029996-119-14-13?v=Kinex+Pharmaceuticals
    Average 90 stars, based on 1 article reviews
    kam-880 microarray kit - by Bioz Stars, 2026-07
    90/100 stars

    Images

    1) Product Images from "N-WASP Attenuates Cell Proliferation and Migration through ERK2-Dependent Enhanced Expression of TXNIP"

    Article Title: N-WASP Attenuates Cell Proliferation and Migration through ERK2-Dependent Enhanced Expression of TXNIP

    Journal: Biology

    doi: 10.3390/biology11040582

    FOXO1 expression is reduced in HSC-5 NW cells. ( A ) Individual duplicate arrays of phospho-Ser319 FOXO1 for the HSC-5 CN (red) and HSC-5 NW (green) cell lysate protein microarrays; the complete Kinex KAM-880 microarray is shown in . The average values of HSC-5 CN and HSC-5 NW cell phospho-Ser319 FOXO1 arrays are shown. ( B ) Representative Western blots of FOXO1 and GAPDH in HSC-5 CN and HSC-5 NW cells; n = 3. ( C ) Densitometric quantification of the FOXO1/GAPDH ratio in HSC-5 CN and HSC-5 NW cells normalized to HSC-5 CN cells. ( D ) Total RNA was extracted from HSC-5 CN and HSC-5 NW cells and reverse-transcribed to cDNA for quantifying FOXO1 expression relative to MRPL-27 using real-time PCR, normalized to HSC-5 CN cells. ( E ) Representative Western blots of FOXO1 and GAPDH in HSC-5 CN and HSC-5 NW cells treated with 10 µM MG132 or DMSO; n = 3. ( F ) Densitometric quantification of the FOXO1/GAPDH ratio in HSC-5 CN and HSC-5 NW cells treated as in ( E ) normalized to DMSO-treated HSC-5 CN cells. ( G ) Representative immunofluorescence images of HSC-5 CN and HSC-5 NW cells treated as in ( E ) stained for FOXO1 (green). Nuclei were counterstained with DAPI (blue). Scale bar represents 20 µm, n = 3. All values are the mean ± SD, n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001.
    Figure Legend Snippet: FOXO1 expression is reduced in HSC-5 NW cells. ( A ) Individual duplicate arrays of phospho-Ser319 FOXO1 for the HSC-5 CN (red) and HSC-5 NW (green) cell lysate protein microarrays; the complete Kinex KAM-880 microarray is shown in . The average values of HSC-5 CN and HSC-5 NW cell phospho-Ser319 FOXO1 arrays are shown. ( B ) Representative Western blots of FOXO1 and GAPDH in HSC-5 CN and HSC-5 NW cells; n = 3. ( C ) Densitometric quantification of the FOXO1/GAPDH ratio in HSC-5 CN and HSC-5 NW cells normalized to HSC-5 CN cells. ( D ) Total RNA was extracted from HSC-5 CN and HSC-5 NW cells and reverse-transcribed to cDNA for quantifying FOXO1 expression relative to MRPL-27 using real-time PCR, normalized to HSC-5 CN cells. ( E ) Representative Western blots of FOXO1 and GAPDH in HSC-5 CN and HSC-5 NW cells treated with 10 µM MG132 or DMSO; n = 3. ( F ) Densitometric quantification of the FOXO1/GAPDH ratio in HSC-5 CN and HSC-5 NW cells treated as in ( E ) normalized to DMSO-treated HSC-5 CN cells. ( G ) Representative immunofluorescence images of HSC-5 CN and HSC-5 NW cells treated as in ( E ) stained for FOXO1 (green). Nuclei were counterstained with DAPI (blue). Scale bar represents 20 µm, n = 3. All values are the mean ± SD, n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001.

    Techniques Used: Expressing, Microarray, Western Blot, Real-time Polymerase Chain Reaction, Immunofluorescence, Staining



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    FOXO1 expression is reduced in HSC-5 NW cells. ( A ) Individual duplicate arrays of phospho-Ser319 FOXO1 for the HSC-5 CN (red) and HSC-5 NW (green) cell lysate protein microarrays; the complete Kinex <t>KAM-880</t> microarray is shown in . The average values of HSC-5 CN and HSC-5 NW cell phospho-Ser319 FOXO1 arrays are shown. ( B ) Representative Western blots of FOXO1 and GAPDH in HSC-5 CN and HSC-5 NW cells; n = 3. ( C ) Densitometric quantification of the FOXO1/GAPDH ratio in HSC-5 CN and HSC-5 NW cells normalized to HSC-5 CN cells. ( D ) Total RNA was extracted from HSC-5 CN and HSC-5 NW cells and reverse-transcribed to cDNA for quantifying FOXO1 expression relative to MRPL-27 using real-time PCR, normalized to HSC-5 CN cells. ( E ) Representative Western blots of FOXO1 and GAPDH in HSC-5 CN and HSC-5 NW cells treated with 10 µM MG132 or DMSO; n = 3. ( F ) Densitometric quantification of the FOXO1/GAPDH ratio in HSC-5 CN and HSC-5 NW cells treated as in ( E ) normalized to DMSO-treated HSC-5 CN cells. ( G ) Representative immunofluorescence images of HSC-5 CN and HSC-5 NW cells treated as in ( E ) stained for FOXO1 (green). Nuclei were counterstained with DAPI (blue). Scale bar represents 20 µm, n = 3. All values are the mean ± SD, n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001.
    Kam 880 Microarray Kit, supplied by Kinex Pharmaceuticals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/kam-880+microarray+kit/pmc09029996-119-14-13?v=Kinex+Pharmaceuticals
    Average 90 stars, based on 1 article reviews
    kam-880 microarray kit - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    FOXO1 expression is reduced in HSC-5 NW cells. ( A ) Individual duplicate arrays of phospho-Ser319 FOXO1 for the HSC-5 CN (red) and HSC-5 NW (green) cell lysate protein microarrays; the complete Kinex KAM-880 microarray is shown in . The average values of HSC-5 CN and HSC-5 NW cell phospho-Ser319 FOXO1 arrays are shown. ( B ) Representative Western blots of FOXO1 and GAPDH in HSC-5 CN and HSC-5 NW cells; n = 3. ( C ) Densitometric quantification of the FOXO1/GAPDH ratio in HSC-5 CN and HSC-5 NW cells normalized to HSC-5 CN cells. ( D ) Total RNA was extracted from HSC-5 CN and HSC-5 NW cells and reverse-transcribed to cDNA for quantifying FOXO1 expression relative to MRPL-27 using real-time PCR, normalized to HSC-5 CN cells. ( E ) Representative Western blots of FOXO1 and GAPDH in HSC-5 CN and HSC-5 NW cells treated with 10 µM MG132 or DMSO; n = 3. ( F ) Densitometric quantification of the FOXO1/GAPDH ratio in HSC-5 CN and HSC-5 NW cells treated as in ( E ) normalized to DMSO-treated HSC-5 CN cells. ( G ) Representative immunofluorescence images of HSC-5 CN and HSC-5 NW cells treated as in ( E ) stained for FOXO1 (green). Nuclei were counterstained with DAPI (blue). Scale bar represents 20 µm, n = 3. All values are the mean ± SD, n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001.

    Journal: Biology

    Article Title: N-WASP Attenuates Cell Proliferation and Migration through ERK2-Dependent Enhanced Expression of TXNIP

    doi: 10.3390/biology11040582

    Figure Lengend Snippet: FOXO1 expression is reduced in HSC-5 NW cells. ( A ) Individual duplicate arrays of phospho-Ser319 FOXO1 for the HSC-5 CN (red) and HSC-5 NW (green) cell lysate protein microarrays; the complete Kinex KAM-880 microarray is shown in . The average values of HSC-5 CN and HSC-5 NW cell phospho-Ser319 FOXO1 arrays are shown. ( B ) Representative Western blots of FOXO1 and GAPDH in HSC-5 CN and HSC-5 NW cells; n = 3. ( C ) Densitometric quantification of the FOXO1/GAPDH ratio in HSC-5 CN and HSC-5 NW cells normalized to HSC-5 CN cells. ( D ) Total RNA was extracted from HSC-5 CN and HSC-5 NW cells and reverse-transcribed to cDNA for quantifying FOXO1 expression relative to MRPL-27 using real-time PCR, normalized to HSC-5 CN cells. ( E ) Representative Western blots of FOXO1 and GAPDH in HSC-5 CN and HSC-5 NW cells treated with 10 µM MG132 or DMSO; n = 3. ( F ) Densitometric quantification of the FOXO1/GAPDH ratio in HSC-5 CN and HSC-5 NW cells treated as in ( E ) normalized to DMSO-treated HSC-5 CN cells. ( G ) Representative immunofluorescence images of HSC-5 CN and HSC-5 NW cells treated as in ( E ) stained for FOXO1 (green). Nuclei were counterstained with DAPI (blue). Scale bar represents 20 µm, n = 3. All values are the mean ± SD, n = 3, * p < 0.05, ** p < 0.01, *** p < 0.001.

    Article Snippet: To identify the signaling pathways altered in HSC-5 NW cells, we used the Kinex KAM-880 microarray kit, which detects 518 pan-specific proteins and 359 phospho-specific proteins critical for cell signaling pathways.

    Techniques: Expressing, Microarray, Western Blot, Real-time Polymerase Chain Reaction, Immunofluorescence, Staining